搜索 - 科创

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做了好久的蛋白实验发现经典转膜的黄金参数是转膜液（10X电转液（SolarBio）*1+甲醇*2+ddH2O*7）*尽量夹在三明治中心的胶和膜（控制并联电路电阻）*300~350mA（限流）*...

王萧等通过western blot法检测caspase-3蛋白水平的表达，结果在凋亡细胞出现比例最大的同时，才出现了激活的caspase-3蛋白。

为了检测蛋白的产量，就要做Western blot。做这个工作，转膜时一般都把浓缩胶剥离扔掉。因为我以前没做过，就把整块胶都转了。压了X光片后发现，LEF-10泳道浓缩胶位置有很强的杂交信号。

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