【Applied Mircobiology & Biotechnology】Atlas Wu
Incubation and Screening of Lignin Degradation Fungi
Adversity Ecology national key Lab, College of Plant Protection, Northwest A&F UniversitySummary.
Ligninolytic enzyme system Call degradate the lignin in lignocellulose materials and further destroy the lignin-hemicellulose-cellulose structure of lignocellulose materials, As a result, cellulose is exposed thus further being processed．Ligninolytic enzyme system is widely used in bio-pulping, bio-bleaching, wastewater treatment et al. because reproducible lignocellulose material-based alcohol production using enzymatic hydrolysis technology is increasingly research focus, ligninolytic enzyme system, as a crucial role in pretreatment process of biotransformation technology of lignocellulose material，raise greatly scientists' research enthusiasm on it. the catalytic mechanisms of three ligninolytic enzymes including manganese peroxidase，lignin peroxidase and laccase were focus on in detail．The current screening and activity evaluation methods on lignolytic enzyme and the species with high yield of lignololytic enzymes screened from nature were summarized. Part 1, Preliminary Screening of lignin degradation fungiIntroduction.
Lignin degradation fungi could product three kinds of enzymes, lignin peroxidase(LiP), manganese peroxidase(MnP), laccase(Lac). LiP and MnP have oxidation ability, they could make the Aniline-Blue(A-B) fade. Incubate fungi with the medium that was added the Aniline-Blue, which was used for identifing of lignin degradation fungi. Materials and Methods
The white rot fungus Phanerochaete chrysosporiurn
(CICC 40719) was used throughout the work and was maintained at room temperature on PDA slants.
Ulminite samples gather from Huodi pond, Qinling Mountains.Medium and Culture Conditions.
Martin medium（A-B）(per liter): 5g Peptone, 10g Dextrose, 1g KH2PO4, 0.5g MgSO4, 15g Agar, 0.2g Aniline-Blue.
[s:9]DA medium（A-B）(per liter): 200g Potato, 15g Dextrose, 15g Agar, 0.2g Aniline-Blue.
Screening were studied in non-agitated cultures (28℃) in Martin medium with Aniline-Blue. The Ulminite samples were pestled with sterile water, and spread on the Martin medium（A-B） and PDA medium（A-B）. Reference Phanerochaete chrysosporiurn
were incubated and treated like the screening cultures.