Incubation & Screening of Lignin Degradation Fungi
atlas 2011-7-28农业
【Applied Mircobiology & Biotechnology】
Incubation and Screening of Lignin Degradation Fungi


Atlas Wu
Adversity Ecology national key Lab, College of Plant Protection, Northwest A&F University


Summary.
       Ligninolytic enzyme system Call degradate the lignin in lignocellulose materials and further destroy the lignin-hemicellulose-cellulose structure of lignocellulose materials, As a result, cellulose is exposed thus further being processed.Ligninolytic enzyme system is widely used in bio-pulping, bio-bleaching, wastewater treatment et al. because reproducible lignocellulose material-based alcohol production using enzymatic hydrolysis technology is increasingly research focus, ligninolytic enzyme system, as a crucial role in pretreatment process of biotransformation technology of lignocellulose material,raise greatly scientists' research enthusiasm on it. the catalytic mechanisms of three ligninolytic enzymes including manganese peroxidase,lignin peroxidase and laccase were focus on in detail.The current screening and activity evaluation methods on lignolytic enzyme and the species with high yield of lignololytic enzymes screened from nature were summarized.

Part 1, Preliminary Screening of lignin degradation fungi

Introduction.  
        Lignin degradation fungi could product three kinds of enzymes, lignin peroxidase(LiP), manganese peroxidase(MnP), laccase(Lac). LiP and MnP have oxidation ability, they could make the Aniline-Blue(A-B) fade. Incubate fungi with the medium that was added the Aniline-Blue, which was used for identifing of lignin degradation fungi.

Materials and Methods

Organism.

        The white rot fungus Phanerochaete chrysosporiurn (CICC 40719) was used throughout the work and was maintained at room temperature on PDA slants.
Ulminite samples gather from Huodi pond, Qinling Mountains.

Medium and Culture Conditions.
        Martin medium(A-B)(per liter): 5g Peptone, 10g Dextrose, 1g KH2PO4, 0.5g MgSO4, 15g Agar, 0.2g Aniline-Blue.
        [s:9]DA medium(A-B)(per liter): 200g Potato, 15g Dextrose, 15g Agar, 0.2g Aniline-Blue.
        Screening were studied in non-agitated cultures (28℃) in Martin medium with Aniline-Blue. The Ulminite samples were pestled with sterile water, and spread on the Martin medium(A-B) and PDA medium(A-B). Reference Phanerochaete chrysosporiurn were incubated and treated like the screening cultures.
+50  科创币    托尼史塔克   2011-07-29   优秀~
+50  科创币    焓熵`   2011-07-29   邪惡英文,看不懂的說~
+50  科创币    wesker   2011-07-29   
来自:农林牧渔 / 农业
4
 
atlas 作者
10年11个月前
1楼
Phanerochaete chrysosporiurn
IMG_0873.jpg
Ulminite samples
IMG_0949.jpg
bechtop
IMG_0920.jpg
Martin medium(Aniline-Blue)
IMG_0944.jpg

Spreading
IMG_0945.jpg
IMG_0946.jpg

Incubation environment
   IMG_0943.jpg
IMG_0947.jpg   
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托尼史塔克
10年11个月前
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Re:Incubation & Screening of Lignin Degradation Fungi
Impressive!
Although I don't know much in this field and I don't have much to comment on...
I just want to say : Wonderful, beautiful, remarkable, so excellent so outstanding so spectacular ...
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atlas作者
10年11个月前
3楼
Preliminary Screening result
IMG_0869.jpg
IMG_0870.jpg   
We see the fade circle. The fungus make the Aniline-Blue fade. So we pickingcolony and inoculate it to the Martin-AB medium
IMG_0894.jpg
IMG_0895.jpg   
According to the feature of this fungus, we judge the fungus belongs to Trichoderma.
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